Journal: The Journal of Comparative Neurology
Article Title: Distribution of Voltage‐Gated Sodium Channels and Scaffolding Proteins on Vestibular Calyx Ending Delineates the Axon Initial Segment
doi: 10.1002/cne.70127
Figure Lengend Snippet: Na V 1.8 antibody labels vestibular afferents and has three different patterns. (A) The heminodes of calyx‐only afferents ( green , color conventions are the same in all panels) are densely labeled with Na V 1.8‐like immunoreactivity ( red ), as seen in both longitudinal section (A) and (B) cross‐section of calyces labeled with calretinin. (C) Occasionally, the inner membrane of a calyx surrounding a type I hair cell, most likely belonging to a dimorphic afferent since these were found in the periphery, is labeled with Na V 1.8. However, because confocal lacks the resolution of electron microscopy, this could also be a type I hair cell membrane. In (D) Na V 1.8 is seen to label the calyx endings of calyces ( arrows ) with relatively light calretinin label (D’). Scale bars: A, C, D = 5 µm, B = 10 µm.
Article Snippet: Na V 1.8 , NeuroMab , 75‐166 RRID:AB_2183861 , , , , X , , Datasheet.
Techniques: Labeling, Membrane, Electron Microscopy